Flatbush is a community in the borough of Brooklyn, in New York City. Despite Brooklyn being mentioned in the various TV shows, books and in the movie, Flatbush was only ever mentioned on The Super Mario Bros. Super Show! and occasionally in issues of Nintendo Comics System.
Flatbush is the neighborhood where Mario and Luigi grew up, and is also where they set up Mario Brothers Plumbing. As such, all the live-action segments take place in Flatbush. A famous street in Flatbush is the eponymously named "Flatbush Avenue".
During return trips to Brooklyn, it is unknown if the neighborhood of Flatbush is actually visited or not. Salvador Drainado claimed to be from Flatbush, and managed to return home after being stuck on the Mushroom Planet for thirteen years in "Brooklyn Bound / Cher's Poochie".
In "Flatbush Koopa / Opera", King Koopa, after the Koopa Pack was forced to disband in the Mushroom Kingdom, Koopa attempted to take over all of Brooklyn. Upon seeing Mario and Luigi, he threatened to flatten them worse than he was going to flatten Flatbush. However, his takeover was ultimately foiled.
In the comic "Beauty and the Beach", it is shown that a yacht owned by Mario is named as The Spirit of Flatbush.