In Donkey Kong 64, they appear as the main objectives of the game and have a Nintendo 64 logo on them. They were stolen from Donkey Kong's Banana Hoard by the Kremlings in order to distract them in preparation of their large machine known as the Blast-O-Matic. They are needed to get B. Locker, the character who blocks the entrances to new worlds, to move out of the way. There are 25 Golden Bananas in every world. Each Kong can only collect 5 each. Making there a total of 200 bananas in the 8 worlds. If the Kongs manage to collect all the Banana Fairies and return to the Banana Fairy Queen, she will unlock a door that allows you to retrieve the 201st Golden Banana. This can be done with Tiny Kong, but there is a glitch where you can do this with Chunky Kong as well. If you get all 201 Golden Bananas in Donkey Kong 64, you can watch the secret ending. In Donkey Kong Country Returns the bannana returns too. You can collect it at the end of the Golden Temple.
- Only one banana in DK64 does not have an N64 logo on it. Instead, the logo is that of Rare.
- A 202nd banana can be obtained through a glitch.